ALT
| Catalog numbers: | ||
| UV 185-X | 1 x 100 mL | R1 |
| 1 x 25 mL | R2 | |
| UV 185-R | 4 x 100 mL | R1 |
| 1 x 100 mL | R2 | |
| 911 Packaging - Contact Synermed | ||
- Liquid-Stable Ready-to-Use
- IFCC Formulation
- Linear to 1,000 U/L
Principles of the Reaction
The Expert Panel of Enzymes, International Federation of Clinical
Chemistry has published its recommendations for the measurement of
serum ALT. The Synermed® method for measuring ALT
activity in serum is based upon these recommendations.
The alanine aminotransferase in the sample catalyzes the transfer of the amino
group from alanine to alpha-ketoglutarate to form pyruvate and glutamate.
ALT L-alanine + α-ketoglutarate ->
pyruvate + glutamate
The pyruvate is reduced to lactate in the presence of lactate dehydrogenase [LDH]
with the concurrent oxidation of reduced nicotinamide adenine
dinucleotide [NADH] to nicotinamide adenine dinucleotide [NAD].
LDH pyruvate + NADH ->
lactate + NAD
The resulting rate of
decrease in absorbance at 340 nm is directly proportional to the ALT activity in the sample.