CK
| Catalog numbers: |
| UV 310-X | 1 x 50 mL | R1 |
| 1 x 25 mL | R2 |
| UV 310-R | 3 x 50 mL | R1 |
| 3 x 25 mL | R2 |
| 911 Packaging - Contact Synermed |
- Extended "On-Board" Stability
- IFCC Formulation
- Linear to 1,000 U/L
Principles of the Reaction
Creatine kinase catalyzes the reversible transfer of a phosphate group from creatine
phosphate to adenosine disphosphate [ADP] forming ATP.
| CK | |
| creatine phosphate + ADP | -> |
creatine + ATP |
The
ATP produced is a substrate for the coupled activities of glucokinase
(GK) and glucose-6-phosphate dehydrogenase [G-6-PDH], resulting in
the reduction of nicotinamide adenine dinucleotide phosphate [NADP+
to NADPH]
| GK | |
| ATP + glucose | -> |
glucose-6-phosphate + ADP |
| G-6-PDH | |
| Glucose-6-phosphate + NADP+ | -> |
6-phosphogluconate + NADPH + H+ |
Conditions are such
that the CK reaction is rate-limiting therefore, the resulting
increase in absorbance at 340 nm is directly proportional to the CK
level in the sample.
The International Federation of Clinical Chemistry has published
recommended conditions for the measurement of CK. The Synermed®
CK method is based upon these recommendations.