γ-GT
| Catalog numbers: | |
| VI 410-X | 2 x 50 mL |
| VI 410-R | 6 x 100 mL |
| 911 Packaging - Contact Synermed | |
- Freely-Soluble Substrate
- IFCC Formulation
- Linear to 1,500 U/L
Principles of the Reaction
The Synermed® γ-GT
procedure utilizes a modification of the method developed by Szasz
wherein γ-GT catalyzes the transfer of the g-glutamyl group from the substrate
g-glutamyl-3-carboxy-4-nitroanilide to glycylglycine yielding
5-amino-2-nitrobenzoate which absorbs at 405-410 nm.
γ-GT γ-glutamyl-3-carboxy-4- nitroanilide + glycylglycine
->
g-glutamyl-glycylglycine + 5-amino-2-nitrobenzoate
The change in absorbance at 405-410 nm due to the formation of the
5-amino-2-nitrobenzoate is directly proportional to the γ-GT
activity in the sample.