Phosphorus
| Catalog numbers: | |
| IR 110-X | 1 x 250 mL |
| 1 x 100 mL | |
| IR 110-R | 3 x 250 mL |
| 3 x 100 mL | |
| 911 Packaging - Contact Synermed | |
- Liquid-Stable Ready-to-Use Reagents
- Minimal Interference from Serum Bilirubin, Hemoglobin and Lipemia
- Avoids Error from Abnormal Proteins
Phosphorus assays have been used to monitor intravenous nutrition in geriatric poulations receiveing Intralipid®. The Intralipid causes severe interference in traditional phosphorus assays providing misleading information.
The Synermed® phosphorus assay avoids interference from abnormal proteins, chromatic substances and other turbidity related interferences by means of kinetic infrared detection.
Principles of the Reaction
Synermed provides liquid, stable reagents for the measurement of phosphorus. In the Synermed method, polyvinylpyrrolidone is used to catalyze the reaction of phosphorus with molybdate. The phospho-molybdate complex is reduced in the second step, producing an intense blue infrared-absorbing chromophore which can be quantitated between 600 nm and 850 nm. The infrared detection of the Synermed method eliminates chromatic interferences from hemolysis, bilirubin, and lipemia. The Synermed method is also free from interference from protein.
Polyvinylpyrrolidone [PVP] catalyzes the formation of the molybdate polymer which reacts with phosphorus to form a phosphomolybdate complex.
| PVP | |
| H3PO4 + MoVI -> | 12-molybdophosphoric acid + H+ |
| (yellow) |
The phosphomolybdate complex is reduced to form an intense infrared absorbing blue chromophore which is quantitated spectrophotometrically between 600 and 850 nm.
| 12-molybdophosphoric acid + e- -> | phosphomolybdenum blue |
Analytical Range
Most applications of this procedure have been found to be linear to at least 20 mg/dL.
Expected Values
The normal range for phosphorus in serum is reported to be 2.3-4.3 mg/dL.
Specific Performance Characteristics
1. The Synermed Phosphorus methodology was correlated to a widely accepted colorimetric method. The calculated linear regression on 50 samples ranging from approximately 1.9 to 9.1 mg/dL with Synermed results on the Y-axis is Y + 0.98X + 0.1 with a correlation coefficient of 0.998.
2. The sensitivity of the procedure is such that an absorbance change of 0.001 will detect as little as 0.02 mg/dL.
3. The within run precision of the method using an automated analyzer was determined by assaying 30 replicate samples of quality control materials at two levels of phosphorus concentrations yielding the following results:
4. The run to run reproducibility of the method as applied to an automated analyzer was determined from the values obtained by 5 replicate analyses of quality control material assayed over a 22 batch period. The following results were obtained:
Mean SD CV 2.6 mg/dL 0.04 1.7% 5.7 mg/dL 0.11 1.9%
Mean SD CV 5.0 mg/dL 0.19 3.6% 6.6 mg/dL 0.12 2.0%