Uric Acid
| Catalog numbers: | ||
| IR 460-X | 1 x 250 mL | R1 |
| 1 x 100 mL | R2 | |
| IR 460-R | 3 x 250 mL | R1 |
| 3 x 100 mL | R2 | |
| 911 Packaging - Contact Synermed | ||
- Liquid-Stable Ready-to-Use Reagents
- Minimal Interference from Serum Bilirubin, Hemoglobin and Lipemia
- Extended "On-Board" Stability
Principles of the Reaction
The Synermed Uric Acid reagent system determines uric acid concentrations enzymatically using uricase and peroxidase. The hydrogen peroxide formed reacts with a near infrared chromogen that is quantitated at 600 nm.
| uricase | ||
| Uric Acid + O2 + H2O | -> | Allantoin + CO2 + H2O2 |
| POD | ||
| H2O2 + H3+O + Azure D-2 | -> | Azure D2 chromophore |
Analytical Range
Most applications ofthe procedure have been found to be linear from 0 to 30 mg/dL [1700 µmol/L] uric acid.
Expected Values
The normal range for uric acid in adults is reported to be:
Males 3.5 - 8.0 mg/dL (210 - 475 µmol/L) Females 2.5 - 6.2 mg/dL (150 - 370 µmol/L)
Specific Performance Characteristics
1. The Synermed uric acid methodology was correlated to a commercially available enzymatic uric acid method. The calculated linear regression on 49 samples ranging from approximately 2 to 10 mg/dL with Synermed results on the Y-axis was Y = 0.94X + 0.04 with a correlation coefficient of 0.998.
2. The sensitivity of the procedure is such that an absorbance change of 0.001 will detect as little as 0.14 mg/dl uric acid.
3. The average within run precision of the method was determined by assaying 35 samples of quality control material at two levels of uric acid concentration:
4. The run to run reproducibility of the method as applied to an automated analyzer was determined from the values obtained by 5 replicate analyses of quality control material assayed over a 23 batch period.
Mean SD CV 5.0 mg/dl (298 µmol/L) 0.05 (3.0) 1.1% 9.6 mg/dl (571 µmol/L) 0.05 (3.0) 0.5%
Mean SD CV 4.8 mg/dl (286 µmol/L) 0.23 (14) 4.8% 9.8 mg/dl (583 µmol/L) 0.39 (23) 4.0%